Toilet training little boys
Little boys all too often miss the toilet. Urine odors around a toilet can be difficult to treat once it soaks into the flooring.
Try pouring a fourth of a cup of Nature's Miracle, found at pet stores, on the area. Let it penetrate to the sub-layers of the flooring. Nature's Miracle is made from enzymes that "eat" the bacteria causing the odors. It does not mask odors like most chemical treatments, it gets rid of the cause of the odors so they cannot return. If this does not solve the problem, then you will need to refinish or replace the floor.
Here is a trick to help your little boy hit the toilet. Place a piece of toilet paper on top of the water. Have him aim for the sheet to "sink" the boat. Most boys love the game and their aim gets pretty good!